Fig. 7
From: Binding of NF-Y to transposable elements in mouse and human cells
RNA production in TEs bound by NF-Y. A. Contingency table of NF-Y binding to repetitive elements and repetitive transcripts in ENCODE K562, GM12878 and HepG2 cells. Transcribed (T) and Bound (B) repeats number higher than the relative expected number in all cases. B. Distribution of transcription levels of transcribed repetitive sequences bound or not bound by NF-YB. C. Comparison of eRNA and not eRNA loci within TEs bound or not bound by NF-Y in K562 (Left Panel), GM12878 (Middle Panel) and HepG2 (Right Panel) cell lines. Statistical significance assessed with Fisher’s exact test (A, C) and Wilcoxon rank-sum test (B). Significance levels are given by stars: * − 10− 2, ** − 10− 3 and *** − 10− 10. D. RT-qPCR quantification of the expression of several LTR regions upon NF-YB siRNA-treatment in HeLa cells. Relative expression was expressed relative to control siRNA treated cells (siCTR). For LTR targets also RT- control results are shown to evaluate genomic DNA carryover. The genomic regions assessed are indicated below the plot. Bar plots correspond to the average of three biological replicas (n = 3). Error bars correspond to the SEM. * pvalue < 0.05 according to one-sample t-test